Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 458529..459100 Replicon chromosome
Accession NZ_AP026566
Organism Enterococcus faecium strain JHP9

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag OQH78_RS02210 Protein ID WP_002286801.1
Coordinates 458759..459100 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag OQH78_RS02205 Protein ID WP_002323011.1
Coordinates 458529..458759 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH78_RS02180 (EfmJHP9_04510) 453662..454993 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
OQH78_RS02185 (EfmJHP9_04520) 455015..455641 + 627 WP_002286816.1 cysteine hydrolase -
OQH78_RS02190 (EfmJHP9_04530) 455824..456405 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
OQH78_RS02195 457137..457699 + 563 Protein_435 SOS response-associated peptidase -
OQH78_RS02200 (EfmJHP9_04560) 457904..458242 - 339 WP_002286804.1 hypothetical protein -
OQH78_RS02205 (EfmJHP9_04570) 458529..458759 + 231 WP_002323011.1 hypothetical protein Antitoxin
OQH78_RS02210 (EfmJHP9_04580) 458759..459100 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH78_RS02215 (EfmJHP9_04590) 459950..460135 + 186 WP_002304207.1 hypothetical protein -
OQH78_RS02220 (EfmJHP9_04610) 460640..460834 + 195 WP_002297028.1 hypothetical protein -
OQH78_RS02225 461025..461274 + 250 Protein_441 transposase -
OQH78_RS02230 (EfmJHP9_04620) 461518..462348 - 831 WP_002286789.1 manganese catalase family protein -
OQH78_RS02235 (EfmJHP9_04630) 462556..463890 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T42549 WP_002286801.1 NZ_AP026566:458759-459100 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T42549 NZ_AP026566:458759-459100 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT42549 WP_002323011.1 NZ_AP026566:458529-458759 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT42549 NZ_AP026566:458529-458759 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References