Detailed information of TA system TA042542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 655910..656469
• Replicon: chromosome
• Accession: NZ_AP026553
• Organism: Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: OO986_RS18165
• Protein ID: WP_108695078.1
• Coordinates: 655910..656188 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3Q0L0M2
• Locus tag: OO986_RS18170
• Protein ID: WP_011082348.1
• Coordinates: 656185..656469 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO986_RS18145 (VV208B2_34280)	650983..651498	+	516	WP_045621343.1	lipocalin family protein	-
OO986_RS18150 (VV208B2_34290)	652108..653610	+	1503	WP_264922980.1	ATP-binding protein	-
OO986_RS18155	654328..655536	+	1209	WP_014386814.1	IS4-like element ISVsa5 family transposase	-
OO986_RS18160	655789..655881	+	93	WP_140142896.1	DUF3265 domain-containing protein	-
OO986_RS18165 (VV208B2_34310)	655910..656188	-	279	WP_108695078.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OO986_RS18170 (VV208B2_34320)	656185..656469	-	285	WP_011082348.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
OO986_RS18175 (VV208B2_34330)	656703..657512	+	810	WP_108678046.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
OO986_RS18180 (VV208B2_34340)	657752..658015	+	264	WP_108695079.1	hypothetical protein	-
OO986_RS18185 (VV208B2_34350)	658093..658593	+	501	WP_108695080.1	opioid growth factor receptor-related protein	-
OO986_RS18190 (VV208B2_34360)	658673..659704	-	1032	WP_011151707.1	L-threonine 3-dehydrogenase	-
OO986_RS18195 (VV208B2_34370)	659749..660942	-	1194	WP_011082341.1	glycine C-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	654328..655536	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-45)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10937.60 Da
• Isoelectric Point: 4.3324

>T42542 WP_108695078.1 NZ_AP026553:c656188-655910 [Vibrio vulnificus]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDNLIETKVENLLEQPLMGVQRDSIRGRLLIIPEISMVISYWVDGDIIRVM
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T42542 NZ_AP026553:c656188-655910 [Vibrio vulnificus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAACAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATAGCA
TCCGCGGTCGATTACTCATTATTCCTGAGATTTCGATGGTTATCTCTTACTGGGTCGATGGCGATATCATCCGAGTTATG
CGTGTCCTCCACCAGAAACAAAAATTCCCAACGGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11083.43 Da
• Isoelectric Point: 9.2167

>AT42542 WP_011082348.1 NZ_AP026553:c656469-656185 [Vibrio vulnificus]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKTLSHDAWLTEQVNLAFEKFDSGKSVFIEHQTAKSR
MEERKARIRNRGKQ

Nucleotide

• Length: 285 bp

>AT42542 NZ_AP026553:c656469-656185 [Vibrio vulnificus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGCAC
TCTTAGTGACGCATGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTGTCTCACGATGCATGGCTAA
CTGAACAAGTAAATCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCATTGAGCACCAAACTGCTAAATCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGGGGTAAGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q0L0M2