Detailed information of TA system TA042540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-Phd
• Location: 1530973..1531520
• Replicon: chromosome
• Accession: NZ_AP026552
• Organism: Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: OO986_RS07285
• Protein ID: WP_000229316.1
• Coordinates: 1531218..1531520 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: OO986_RS07280
• Protein ID: WP_000861987.1
• Coordinates: 1530973..1531230 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO986_RS07240	1526324..1526452	+	129	WP_108695296.1	DUF3265 domain-containing protein	-
OO986_RS07245	1527095..1527220	+	126	WP_001900195.1	DUF645 family protein	-
OO986_RS07250 (VV208B2_13210)	1527469..1527927	+	459	WP_025818672.1	hypothetical protein	-
OO986_RS07255 (VV208B2_13220)	1528037..1528360	+	324	WP_108695200.1	hypothetical protein	-
OO986_RS07260	1528851..1528973	+	123	WP_108695203.1	DUF3265 domain-containing protein	-
OO986_RS07265 (VV208B2_13230)	1528999..1529901	+	903	WP_108695201.1	hypothetical protein	-
OO986_RS07270	1529921..1530012	+	92	Protein_1376	DUF3265 domain-containing protein	-
OO986_RS07275 (VV208B2_13240)	1530042..1530782	+	741	WP_108695202.1	HNH endonuclease	-
OO986_RS07280 (VV208B2_13250)	1530973..1531230	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OO986_RS07285 (VV208B2_13260)	1531218..1531520	+	303	WP_000229316.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OO986_RS07290	1532040..1532168	+	129	Protein_1380	DUF3265 domain-containing protein	-
OO986_RS07295	1532161..1532343	+	183	WP_101957108.1	hypothetical protein	-
OO986_RS07300	1532494..1532622	+	129	Protein_1382	DUF3265 domain-containing protein	-
OO986_RS07305	1533414..1533549	+	136	Protein_1383	DUF3265 domain-containing protein	-
OO986_RS07310	1534934..1535062	+	129	Protein_1384	DUF3265 domain-containing protein	-
OO986_RS07315 (VV208B2_13280)	1535545..1535976	+	432	WP_101957106.1	hypothetical protein	-
OO986_RS07320	1535958..1536093	+	136	Protein_1386	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1460130..1549030	88900
	-	inside	Integron	-	-	1460130..1541709	81579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11753.71 Da
• Isoelectric Point: 5.1972

>T42540 WP_000229316.1 NZ_AP026552:1531218-1531520 [Vibrio vulnificus]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVNPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T42540 NZ_AP026552:1531218-1531520 [Vibrio vulnificus]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT42540 WP_000861987.1 NZ_AP026552:1530973-1531230 [Vibrio vulnificus]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT42540 NZ_AP026552:1530973-1531230 [Vibrio vulnificus]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1T4SLM9