Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3966091..3966710 Replicon chromosome
Accession NZ_AP026525
Organism Klebsiella pneumoniae subsp. pneumoniae strain TUM9839

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag OO859_RS19380 Protein ID WP_002892050.1
Coordinates 3966492..3966710 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag OO859_RS19375 Protein ID WP_002892066.1
Coordinates 3966091..3966465 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO859_RS19365 (TUM9839_37340) 3961243..3962436 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
OO859_RS19370 (TUM9839_37350) 3962459..3965605 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
OO859_RS19375 (TUM9839_37360) 3966091..3966465 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
OO859_RS19380 (TUM9839_37370) 3966492..3966710 + 219 WP_002892050.1 HHA domain-containing protein Toxin
OO859_RS19385 (TUM9839_37380) 3966869..3967435 + 567 WP_002892030.1 maltose O-acetyltransferase -
OO859_RS19390 3967407..3967547 - 141 WP_004147370.1 hypothetical protein -
OO859_RS19395 (TUM9839_37390) 3967568..3968038 + 471 WP_002892026.1 YlaC family protein -
OO859_RS19400 (TUM9839_37400) 3968013..3969464 - 1452 WP_032436946.1 PLP-dependent aminotransferase family protein -
OO859_RS19405 (TUM9839_37410) 3969565..3970263 + 699 WP_002892021.1 GNAT family protein -
OO859_RS19410 (TUM9839_37420) 3970260..3970400 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
OO859_RS19415 (TUM9839_37430) 3970400..3970663 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42532 WP_002892050.1 NZ_AP026525:3966492-3966710 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T42532 NZ_AP026525:3966492-3966710 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT42532 WP_002892066.1 NZ_AP026525:3966091-3966465 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT42532 NZ_AP026525:3966091-3966465 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References