Detailed information of TA system TA042517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 15936..16471
• Replicon: plasmid pKAM645_6
• Accession: NZ_AP026426
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: OCV84_RS30035
• Protein ID: WP_016531292.1
• Coordinates: 15936..16223 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A837JUA1
• Locus tag: OCV84_RS30040
• Protein ID: WP_023317799.1
• Coordinates: 16220..16471 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCV84_RS30015 (KAM645c_58500)	10980..11807	+	828	WP_101527419.1	replication initiation protein	-
OCV84_RS30020 (KAM645c_58520)	13062..13592	+	531	WP_087796343.1	hypothetical protein	-
OCV84_RS30025 (KAM645c_58530)	13849..14136	-	288	WP_261902547.1	stability/ partitioning determinant	-
OCV84_RS30030 (KAM645c_58540)	14226..14888	-	663	WP_016529485.1	division plane positioning ATPase MipZ	-
OCV84_RS30035 (KAM645c_58560)	15936..16223	-	288	WP_016531292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCV84_RS30040 (KAM645c_58570)	16220..16471	-	252	WP_023317799.1	hypothetical protein	Antitoxin
OCV84_RS30045 (KAM645c_58580)	16567..17838	-	1272	WP_261902548.1	Y-family DNA polymerase	-
OCV84_RS30050 (KAM645c_58590)	17838..18263	-	426	WP_072122712.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
OCV84_RS30055 (KAM645c_58600)	18340..19827	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
OCV84_RS30060 (KAM645c_58610)	20439..20663	+	225	WP_087796345.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57457	57457

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11120.16 Da
• Isoelectric Point: 10.4373

>T42517 WP_016531292.1 NZ_AP026426:c16223-15936 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MTYTVKFREDALKEWNKLDKTIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNQLIIAIVAVGK
RERSDVYTLASERMK

Nucleotide

• Length: 288 bp

>T42517 NZ_AP026426:c16223-15936 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACATATACCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGAACAAGCTGGATAAGACTATTCAGCAGCAGTTTGC
AAAGAAACTTAAAAAGTGCTGTGAAAACCCACACATTCCTTCAGCAAAACTACGTGGTATAAAGGATTGCTATAAAATAA
AACTGCGAGCATCTGGTTTTCGCCTCGTCTATCAGGTCATAGATAATCAACTTATAATTGCTATTGTGGCTGTAGGCAAA
CGGGAGCGAAGTGACGTTTATACACTGGCGAGTGAACGTATGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.60 Da
• Isoelectric Point: 4.2108

>AT42517 WP_023317799.1 NZ_AP026426:c16471-16220 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELIKLVAERSNQPLFDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT42517 NZ_AP026426:c16471-16220 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCCACATATCATTCTGAGTGACACGACTGCCAGCGTCAGTGAATTGAAAAAAAACCCGATGGCCACAGTCAGCGCAGG
TGACGGGTTCCCAGTAGCGATTCTTAACCGGAACCAGCCAGCTTTCTATTGCGTGCCTGCGGAACTCTACGAAAAGATGC
TTGATGCGCTGGATGATCAGGAACTCATTAAACTGGTTGCAGAACGCAGTAATCAACCGCTGTTTGATGTGGACCTGGAT
AAGTACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A837JUA1