Detailed information of TA system TA042514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4825..5561
• Replicon: plasmid pKAM645_3
• Accession: NZ_AP026423
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: OCV84_RS28105
• Protein ID: WP_003026803.1
• Coordinates: 5079..5561 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: OCV84_RS28100
• Protein ID: WP_003026799.1
• Coordinates: 4825..5091 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCV84_RS28075 (KAM645c_54640)	1..1011	+	1011	WP_000200070.1	RepB family plasmid replication initiator protein	-
OCV84_RS28080 (KAM645c_54650)	1709..2449	-	741	WP_001515717.1	tyrosine-type recombinase/integrase	-
OCV84_RS28085 (KAM645c_54660)	2813..3781	-	969	WP_023307223.1	IS5-like element IS903B family transposase	-
OCV84_RS28090 (KAM645c_54670)	3933..4136	+	204	WP_004213596.1	HHA domain-containing protein	-
OCV84_RS28095 (KAM645c_54680)	4150..4380	+	231	WP_023307222.1	hypothetical protein	-
OCV84_RS28100 (KAM645c_54690)	4825..5091	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
OCV84_RS28105	5079..5561	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
OCV84_RS28110 (KAM645c_54710)	5762..7165	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
OCV84_RS28115 (KAM645c_54720)	7194..7826	-	633	WP_001567369.1	hypothetical protein	-
OCV84_RS28120	8052..9398	+	1347	WP_261902533.1	ISNCY family transposase	-
OCV84_RS28125 (KAM645c_54750)	9447..9842	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..180281	180281
	-	inside	IScluster/Tn	-	-	2813..12437	9624

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T42514 WP_003026803.1 NZ_AP026423:5079-5561 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T42514 NZ_AP026423:5079-5561 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT42514 WP_003026799.1 NZ_AP026423:4825-5091 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT42514 NZ_AP026423:4825-5091 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66