Detailed information of TA system TA042513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 115300..116923
• Replicon: plasmid pKAM645_2
• Accession: NZ_AP026422
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: OCV84_RS27580
• Protein ID: WP_047744996.1
• Coordinates: 115300..116625 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: OCV84_RS27585
• Protein ID: WP_032636197.1
• Coordinates: 116630..116923 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCV84_RS27550 (KAM645c_53580)	111283..112140	-	858	WP_047744993.1	HNH endonuclease signature motif containing protein	-
OCV84_RS27555 (KAM645c_53590)	112156..112464	-	309	WP_001224686.1	hypothetical protein	-
OCV84_RS27560 (KAM645c_53600)	113015..113440	-	426	WP_001043851.1	hypothetical protein	-
OCV84_RS27565 (KAM645c_53610)	113694..114509	-	816	WP_047744994.1	HNH endonuclease signature motif containing protein	-
OCV84_RS27570 (KAM645c_53620)	114522..114932	-	411	WP_047744995.1	hypothetical protein	-
OCV84_RS27575 (KAM645c_53630)	115033..115239	-	207	WP_000134171.1	hypothetical protein	-
OCV84_RS27580 (KAM645c_53640)	115300..116625	-	1326	WP_047744996.1	type II toxin-antitoxin system HipA family toxin	Toxin
OCV84_RS27585 (KAM645c_53650)	116630..116923	-	294	WP_032636197.1	helix-turn-helix transcriptional regulator	Antitoxin
OCV84_RS27590 (KAM645c_53660)	117322..118710	-	1389	WP_047744997.1	hypothetical protein	-
OCV84_RS27595 (KAM645c_53670)	118715..119212	-	498	WP_047745035.1	hypothetical protein	-
OCV84_RS27600 (KAM645c_53680)	119416..120063	-	648	WP_047744998.1	hypothetical protein	-
OCV84_RS27605 (KAM645c_53690)	120969..121361	-	393	WP_047744999.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / qnrA1 / sul1 / dfrA19 / aph(3'')-Ib / aph(6)-Id	-	1..219538	219538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50155.10 Da
• Isoelectric Point: 6.1413

>T42513 WP_047744996.1 NZ_AP026422:c116625-115300 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKCHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAEHDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPPQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T42513 NZ_AP026422:c116625-115300 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGACGAACCCACAGATTTATATTCCGTGAAGTGTCACCCTCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAATGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGCATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCACTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGCGCAGAGC
ATGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCAATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACGGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGAGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCCGCAGCTTGCCGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10989.46 Da
• Isoelectric Point: 10.5348

>AT42513 WP_032636197.1 NZ_AP026422:c116923-116630 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTTSSERK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT42513 NZ_AP026422:c116923-116630 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA