Detailed information of TA system TA042512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 144490..145012
• Replicon: plasmid pKAM645_1
• Accession: NZ_AP026421
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: OCV84_RS26535
• Protein ID: WP_004181778.1
• Coordinates: 144728..145012 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: OCV84_RS26530
• Protein ID: WP_004181777.1
• Coordinates: 144490..144738 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCV84_RS26510 (KAM645c_51420)	139699..140520	-	822	WP_004181772.1	hypothetical protein	-
OCV84_RS26515 (KAM645c_51430)	140582..140935	-	354	WP_004181774.1	hypothetical protein	-
OCV84_RS26520 (KAM645c_51440)	141080..142066	-	987	WP_025368599.1	hypothetical protein	-
OCV84_RS26525 (KAM645c_51460)	142400..144199	-	1800	WP_004181776.1	ATP-dependent helicase	-
OCV84_RS26530 (KAM645c_51470)	144490..144738	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OCV84_RS26535 (KAM645c_51480)	144728..145012	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCV84_RS26540	145029..145130	-	102	Protein_154	IS200/IS605 family transposase	-
OCV84_RS26545 (KAM645c_51490)	145166..146392	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
OCV84_RS26550 (KAM645c_51500)	146663..146887	-	225	Protein_156	transposase	-
OCV84_RS26555 (KAM645c_51510)	146966..147394	-	429	WP_077257669.1	IS200/IS605 family transposase	-
OCV84_RS26560 (KAM645c_51520)	147430..148617	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
OCV84_RS26565 (KAM645c_51530)	148662..149033	-	372	WP_040209644.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..224497	224497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T42512 WP_004181778.1 NZ_AP026421:144728-145012 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T42512 NZ_AP026421:144728-145012 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT42512 WP_004181777.1 NZ_AP026421:144490-144738 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT42512 NZ_AP026421:144490-144738 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8