Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4006629..4007247 Replicon chromosome
Accession NZ_AP026420
Organism Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag OCV84_RS19600 Protein ID WP_002892050.1
Coordinates 4007029..4007247 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag OCV84_RS19595 Protein ID WP_002892066.1
Coordinates 4006629..4007003 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OCV84_RS19585 (4001784) 4001784..4002977 + 1194 WP_023288645.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
OCV84_RS19590 (4003000) 4003000..4006146 + 3147 WP_065882810.1 multidrug efflux RND transporter permease subunit AcrB -
OCV84_RS19595 (4006629) 4006629..4007003 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
OCV84_RS19600 (4007029) 4007029..4007247 + 219 WP_002892050.1 HHA domain-containing protein Toxin
OCV84_RS19605 (4007406) 4007406..4007972 + 567 WP_261901706.1 maltose O-acetyltransferase -
OCV84_RS19610 (4007953) 4007953..4008075 - 123 WP_261901707.1 hypothetical protein -
OCV84_RS19615 (4008109) 4008109..4008579 + 471 WP_023288642.1 YlaC family protein -
OCV84_RS19620 (4008548) 4008548..4010005 - 1458 WP_261901708.1 PLP-dependent aminotransferase family protein -
OCV84_RS19625 (4010106) 4010106..4010804 + 699 WP_261901709.1 GNAT family protein -
OCV84_RS19630 (4010801) 4010801..4010941 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
OCV84_RS19635 (4010941) 4010941..4011204 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42510 WP_002892050.1 NZ_AP026420:4007029-4007247 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T42510 NZ_AP026420:4007029-4007247 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGCCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT42510 WP_002892066.1 NZ_AP026420:4006629-4007003 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT42510 NZ_AP026420:4006629-4007003 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACGTTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGATACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References