Detailed information of TA system TA042508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2673504..2675092
• Replicon: chromosome
• Accession: NZ_AP026420
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: OCV84_RS13240
• Protein ID: WP_044523012.1
• Coordinates: 2673504..2674826 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: OCV84_RS13245
• Protein ID: WP_023289667.1
• Coordinates: 2674826..2675092 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCV84_RS13215 (2668550)	2668550..2670205	-	1656	WP_087776238.1	EAL domain-containing protein	-
OCV84_RS13220 (2670503)	2670503..2670751	+	249	WP_004205912.1	hypothetical protein	-
OCV84_RS13225 (2671043)	2671043..2671261	+	219	WP_002904808.1	ParD-like family protein	-
OCV84_RS13230 (2671242)	2671242..2672024	+	783	WP_218810086.1	type I methionyl aminopeptidase	-
OCV84_RS13235 (2672195)	2672195..2673487	+	1293	WP_261901442.1	glycoside hydrolase family 10 protein	-
OCV84_RS13240 (2673504)	2673504..2674826	-	1323	WP_044523012.1	type II toxin-antitoxin system HipA family toxin	Toxin
OCV84_RS13245 (2674826)	2674826..2675092	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
OCV84_RS13250 (2675356)	2675356..2675682	-	327	WP_217332803.1	hydroxyisourate hydrolase	-
OCV84_RS13255 (2675679)	2675679..2676179	-	501	WP_261901443.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
OCV84_RS13260 (2676157)	2676157..2677536	-	1380	WP_261901444.1	nucleobase:cation symporter-2 family protein	-
OCV84_RS13265 (2677882)	2677882..2679036	+	1155	WP_023289663.1	FAD-dependent urate hydroxylase HpxO	-
OCV84_RS13270 (2679017)	2679017..2679940	-	924	WP_241516047.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49183.49 Da
• Isoelectric Point: 8.6110

>T42508 WP_044523012.1 NZ_AP026420:c2674826-2673504 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T42508 NZ_AP026420:c2674826-2673504 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGACGAGTGGCTGCGCAGCCCGCGCGCCAGGCCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCACAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAACAGCCGTTCGATCTGCTGGCTGAGGTCGGCCGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCATCTTGCAGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAACTGACCGCGTTGCTGACCGCCTACCAGT
CGGATATTCCGCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATTGGCGAGAT
AAAACAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTAGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCACCACCCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGGCTGCCGCAAGAGGATCTGTGTCAGGCATTCGGCATCCCGTCGTCGATGAAATA
CGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAATTTCTCGCTCTTTCTACTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGAGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAACGCCACCCGAGGGCGGAAAACGGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTTCCCCGGGATCAGATGCTGGCGATCCTTGACGAGTTTGCCGACCGCGTGCCA
CAGGCCATCGAAAGCGCCCGCCGGACACTGCCGGCGGACTTTTCCGCCCACGTCTGGCGGGCGATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT42508 WP_023289667.1 NZ_AP026420:c2675092-2674826 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT42508 NZ_AP026420:c2675092-2674826 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCATTCCCCTCAGATATACAGTCCCGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCGGACAAAACCACCTTGA
CCACCCTATTCAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCCGAGACGGCGACGGCCCCC
GATAACACCACGGATCTGGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3