Detailed information of TA system TA042498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 58646..59289
• Replicon: plasmid pKAM644_3
• Accession: NZ_AP026410
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: P4I75_RS28445
• Protein ID: WP_049014623.1
• Coordinates: 58873..59289 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: P4I75_RS28440
• Protein ID: WP_001261276.1
• Coordinates: 58646..58876 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I75_RS28430 (KAM644c_55210)	54652..56847	+	2196	WP_049013984.1	DEAD/DEAH box helicase	-
P4I75_RS28435	57167..58327	-	1161	WP_064171855.1	IS30-like element ISCfr4 family transposase	-
P4I75_RS28440 (KAM644c_55230)	58646..58876	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
P4I75_RS28445 (KAM644c_55240)	58873..59289	+	417	WP_049014623.1	type II toxin-antitoxin system VapC family toxin	Toxin
P4I75_RS28455 (KAM644c_55270)	62095..63192	-	1098	WP_049015454.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..220520	220520
	-	inside	IScluster/Tn	-	-	57167..78656	21489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15098.57 Da
• Isoelectric Point: 8.5403

>T42498 WP_049014623.1 NZ_AP026410:58873-59289 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRFAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T42498 NZ_AP026410:58873-59289 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTGGATGCCACGACGGACATCCGGGTGGCGCTGCGCTTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCGGCGCAGTCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT42498 WP_001261276.1 NZ_AP026410:58646-58876 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT42498 NZ_AP026410:58646-58876 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023