Detailed information of TA system TA042496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 117879..119502
• Replicon: plasmid pKAM644_2
• Accession: NZ_AP026409
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: P4I75_RS27600
• Protein ID: WP_276097478.1
• Coordinates: 118177..119502 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: P4I75_RS27595
• Protein ID: WP_032636197.1
• Coordinates: 117879..118172 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I75_RS27575 (KAM644c_53510)	113441..113833	+	393	WP_047744999.1	hypothetical protein	-
P4I75_RS27580 (KAM644c_53520)	114739..115386	+	648	WP_047744998.1	hypothetical protein	-
P4I75_RS27585 (KAM644c_53530)	115590..116087	+	498	WP_047745035.1	hypothetical protein	-
P4I75_RS27590 (KAM644c_53540)	116092..117480	+	1389	WP_047744997.1	hypothetical protein	-
P4I75_RS27595 (KAM644c_53550)	117879..118172	+	294	WP_032636197.1	helix-turn-helix transcriptional regulator	Antitoxin
P4I75_RS27600 (KAM644c_53560)	118177..119502	+	1326	WP_276097478.1	type II toxin-antitoxin system HipA family toxin	Toxin
P4I75_RS27605 (KAM644c_53570)	119563..119769	+	207	WP_000134171.1	hypothetical protein	-
P4I75_RS27610 (KAM644c_53580)	119870..120232	+	363	WP_276097479.1	hypothetical protein	-
P4I75_RS27620 (KAM644c_53610)	121645..122460	+	816	WP_047744994.1	HNH endonuclease signature motif containing protein	-
P4I75_RS27625 (KAM644c_53620)	122714..123139	+	426	WP_001043851.1	hypothetical protein	-
P4I75_RS27630 (KAM644c_53630)	123690..123998	+	309	WP_001224686.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / qnrA1 / sul1 / dfrA19 / aph(3'')-Ib / aph(6)-Id	-	1..222214	222214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50171.14 Da
• Isoelectric Point: 6.1413

>T42496 WP_276097478.1 NZ_AP026409:118177-119502 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKCHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAEHDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWLVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPPQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T42496 NZ_AP026409:118177-119502 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGACGAACCCACAGATTTATATTCCGTGAAGTGTCACCCTCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAATGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGCATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCACTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGCGCAGAGC
ATGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCTGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCAATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACGGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGAGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCCGCAGCTTGCCGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10989.46 Da
• Isoelectric Point: 10.5348

>AT42496 WP_032636197.1 NZ_AP026409:117879-118172 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTTSSERK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT42496 NZ_AP026409:117879-118172 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA