42495

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 142605..143127 Replicon plasmid pKAM644_1
Accession NZ_AP026408
Organism Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag P4I75_RS26595 Protein ID WP_004181778.1
Coordinates 142843..143127 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag P4I75_RS26590 Protein ID WP_004181777.1
Coordinates 142605..142853 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P4I75_RS26575 (KAM644c_51420) 137844..138830 - 987 WP_025368599.1 hypothetical protein -
P4I75_RS26585 (KAM644c_51460) 140515..142314 - 1800 WP_004181776.1 ATP-dependent helicase -
P4I75_RS26590 (KAM644c_51470) 142605..142853 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
P4I75_RS26595 (KAM644c_51480) 142843..143127 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P4I75_RS26600 143144..143245 - 102 Protein_155 IS200/IS605 family transposase -
P4I75_RS26605 (KAM644c_51490) 143281..144507 + 1227 WP_040209639.1 RNA-guided endonuclease TnpB family protein -
P4I75_RS26610 (KAM644c_51500) 144778..145002 - 225 Protein_157 transposase -
P4I75_RS26615 (KAM644c_51510) 145081..145509 - 429 WP_077257669.1 IS200/IS605 family transposase -
P4I75_RS26620 (KAM644c_51520) 145545..146732 + 1188 WP_202162051.1 RNA-guided endonuclease TnpB family protein -
P4I75_RS26625 (KAM644c_51530) 146777..147148 - 372 WP_040209644.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..223942 223942
- inside IScluster/Tn - - 139031..152013 12982


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T42495 WP_004181778.1 NZ_AP026408:142843-143127 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T42495 NZ_AP026408:142843-143127 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT42495 WP_004181777.1 NZ_AP026408:142605-142853 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT42495 NZ_AP026408:142605-142853 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References