Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4005452..4006070 Replicon chromosome
Accession NZ_AP026407
Organism Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag P4I75_RS19610 Protein ID WP_002892050.1
Coordinates 4005852..4006070 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag P4I75_RS19605 Protein ID WP_002892066.1
Coordinates 4005452..4005826 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P4I75_RS19595 (4000607) 4000607..4001800 + 1194 WP_023288645.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
P4I75_RS19600 (4001823) 4001823..4004969 + 3147 WP_065882810.1 multidrug efflux RND transporter permease subunit AcrB -
P4I75_RS19605 (4005452) 4005452..4005826 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
P4I75_RS19610 (4005852) 4005852..4006070 + 219 WP_002892050.1 HHA domain-containing protein Toxin
P4I75_RS19615 (4006229) 4006229..4006795 + 567 WP_261901706.1 maltose O-acetyltransferase -
P4I75_RS19620 (4006776) 4006776..4006898 - 123 WP_261901707.1 hypothetical protein -
P4I75_RS19625 (4006932) 4006932..4007402 + 471 WP_023288642.1 YlaC family protein -
P4I75_RS19630 (4007371) 4007371..4008828 - 1458 WP_261901708.1 PLP-dependent aminotransferase family protein -
P4I75_RS19635 (4008929) 4008929..4009627 + 699 WP_261901709.1 GNAT family protein -
P4I75_RS19640 (4009624) 4009624..4009764 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
P4I75_RS19645 (4009764) 4009764..4010027 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42493 WP_002892050.1 NZ_AP026407:4005852-4006070 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T42493 NZ_AP026407:4005852-4006070 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGCCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT42493 WP_002892066.1 NZ_AP026407:4005452-4005826 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT42493 NZ_AP026407:4005452-4005826 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACGTTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGATACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References