Detailed information of TA system TA042481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 22128..22599
• Replicon: plasmid pKAM643_3
• Accession: NZ_AP026406
• Organism: Aeromonas caviae strain KAM643

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: OCX50_RS23300
• Protein ID: WP_139742234.1
• Coordinates: 22336..22599 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A261E9D6
• Locus tag: OCX50_RS23295
• Protein ID: WP_039216019.1
• Coordinates: 22128..22352 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX50_RS23250 (KAM643c_44850)	17732..17950	-	219	WP_244057344.1	hypothetical protein	-
OCX50_RS23255	18044..18292	+	249	WP_139742226.1	hypothetical protein	-
OCX50_RS23260 (KAM643c_44860)	18506..18955	+	450	WP_139742227.1	hypothetical protein	-
OCX50_RS23265 (KAM643c_44870)	19061..19975	+	915	WP_193944749.1	hypothetical protein	-
OCX50_RS23270 (KAM643c_44880)	19962..20237	+	276	WP_204792479.1	hypothetical protein	-
OCX50_RS23275 (KAM643c_44890)	20256..20564	+	309	WP_139742230.1	hypothetical protein	-
OCX50_RS23280 (KAM643c_44900)	20654..21265	+	612	WP_139742231.1	hypothetical protein	-
OCX50_RS23285 (KAM643c_44910)	21577..21831	+	255	WP_139742233.1	HigA family addiction module antitoxin	-
OCX50_RS23290 (KAM643c_44920)	21867..22052	+	186	Protein_24	helix-turn-helix domain-containing protein	-
OCX50_RS23295 (KAM643c_44930)	22128..22352	+	225	WP_039216019.1	TraY domain-containing protein	Antitoxin
OCX50_RS23300 (KAM643c_44940)	22336..22599	+	264	WP_139742234.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCX50_RS23305 (KAM643c_44950)	22975..23169	-	195	WP_244057346.1	hypothetical protein	-
OCX50_RS23310 (KAM643c_44970)	23210..24243	-	1034	Protein_28	IS630-like element ISAhy2 family transposase	-
OCX50_RS23315 (KAM643c_44990)	24660..24884	-	225	WP_139742236.1	hypothetical protein	-
OCX50_RS23320 (KAM643c_45000)	24947..25273	-	327	WP_139742237.1	hypothetical protein	-
OCX50_RS23325 (KAM643c_45010)	25759..27033	+	1275	WP_244057218.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31284	31284
	-	flank	IS/Tn	-	-	16325..17305	980
	-	flank	IS/Tn	-	-	23467..24243	776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

• Relaxosome protein TraY (4-47)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10280.00 Da
• Isoelectric Point: 9.9650

>T42481 WP_139742234.1 NZ_AP026406:22336-22599 [Aeromonas caviae]
MAWKVELTDTAKKQLAKLDKTQAQRITKYLRRVMMLEDPRDVGKALTGNFRTYWRYRVGDYRVICEIRDDELVIVAVMIG
HRSEVYG

Nucleotide

• Length: 264 bp

>T42481 NZ_AP026406:22336-22599 [Aeromonas caviae]
TTGGCTTGGAAAGTTGAACTGACCGATACAGCCAAGAAGCAGTTGGCAAAATTAGACAAGACACAAGCCCAGCGGATCAC
CAAGTATTTGCGGCGGGTGATGATGCTGGAAGATCCGAGGGATGTAGGCAAAGCATTAACTGGCAATTTCCGAACGTACT
GGCGTTATCGGGTAGGGGATTACCGTGTGATATGTGAGATCCGGGATGATGAACTGGTGATTGTGGCAGTGATGATCGGG
CATAGAAGCGAGGTATATGGGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8664.81 Da
• Isoelectric Point: 4.3736

>AT42481 WP_039216019.1 NZ_AP026406:22128-22352 [Aeromonas caviae]
MLAIRLPSEIEERLAHLAQETGRTKTFYAREAILTHLEELEDYYLAERVMEKVRKGEEPLFSLDDVERDLGLES

Nucleotide

• Length: 225 bp

>AT42481 NZ_AP026406:22128-22352 [Aeromonas caviae]
ATGTTAGCGATTCGCTTGCCTTCGGAGATCGAAGAGCGGTTGGCGCACCTAGCGCAAGAGACGGGAAGAACCAAGACGTT
CTATGCCCGTGAAGCCATTCTGACTCACCTGGAAGAGCTGGAGGATTACTATCTGGCCGAGCGGGTGATGGAAAAGGTTC
GCAAGGGAGAAGAGCCCCTGTTCTCGTTAGATGACGTGGAGCGTGACCTTGGCTTGGAAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A261E9D6