Detailed information of TA system TA042477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 37641..38284
• Replicon: plasmid pKAM622_3
• Accession: NZ_AP026395
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: P4I30_RS29085
• Protein ID: WP_048295728.1
• Coordinates: 37641..38057 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: P4I30_RS29090
• Protein ID: WP_001261276.1
• Coordinates: 38054..38284 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I30_RS29065 (KAM622c_57070)	32861..33295	-	435	WP_001567367.1	cell envelope integrity protein TolA	-
P4I30_RS29070	33342..33841	-	500	Protein_34	thioredoxin fold domain-containing protein	-
P4I30_RS29075 (KAM622c_57100)	34342..34692	+	351	WP_167878626.1	hypothetical protein	-
P4I30_RS29080 (KAM622c_57110)	35333..37471	+	2139	WP_048295729.1	AAA family ATPase	-
P4I30_RS29085 (KAM622c_57120)	37641..38057	-	417	WP_048295728.1	type II toxin-antitoxin system VapC family toxin	Toxin
P4I30_RS29090 (KAM622c_57130)	38054..38284	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
P4I30_RS29095 (KAM622c_57140)	38647..40608	-	1962	WP_276086040.1	tetratricopeptide repeat protein	-
P4I30_RS29100	41455..42152	+	698	WP_276086041.1	IS1 family transposase	-
P4I30_RS29105 (KAM622c_57180)	42429..42608	-	180	WP_276086042.1	hypothetical protein	-
P4I30_RS29110 (KAM622c_57190)	42805..43083	+	279	WP_048295725.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF	1..129931	129931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15094.58 Da
• Isoelectric Point: 8.5403

>T42477 WP_048295728.1 NZ_AP026395:c38057-37641 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPALVLEDWVK

Nucleotide

• Length: 417 bp

>T42477 NZ_AP026395:c38057-37641 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCTGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGCCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT42477 WP_001261276.1 NZ_AP026395:c38284-38054 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT42477 NZ_AP026395:c38284-38054 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023