Detailed information of TA system TA042476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 117549..119172
• Replicon: plasmid pKAM622_2
• Accession: NZ_AP026394
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: P4I30_RS28415
• Protein ID: WP_047744996.1
• Coordinates: 117549..118874 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: P4I30_RS28420
• Protein ID: WP_032636197.1
• Coordinates: 118879..119172 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I30_RS28385 (KAM622c_55580)	113532..114389	-	858	WP_276085935.1	HNH endonuclease signature motif containing protein	-
P4I30_RS28390 (KAM622c_55590)	114405..114713	-	309	WP_001224686.1	hypothetical protein	-
P4I30_RS28395 (KAM622c_55600)	115264..115689	-	426	WP_001043851.1	hypothetical protein	-
P4I30_RS28400 (KAM622c_55610)	115943..116758	-	816	WP_047744994.1	HNH endonuclease signature motif containing protein	-
P4I30_RS28405 (KAM622c_55620)	116771..117181	-	411	WP_047744995.1	hypothetical protein	-
P4I30_RS28410 (KAM622c_55630)	117282..117488	-	207	WP_000134171.1	hypothetical protein	-
P4I30_RS28415 (KAM622c_55640)	117549..118874	-	1326	WP_047744996.1	type II toxin-antitoxin system HipA family toxin	Toxin
P4I30_RS28420 (KAM622c_55650)	118879..119172	-	294	WP_032636197.1	helix-turn-helix transcriptional regulator	Antitoxin
P4I30_RS28425 (KAM622c_55660)	119571..120959	-	1389	WP_276085936.1	hypothetical protein	-
P4I30_RS28430 (KAM622c_55670)	120964..121461	-	498	WP_047745035.1	hypothetical protein	-
P4I30_RS28435 (KAM622c_55680)	121665..122312	-	648	WP_047744998.1	hypothetical protein	-
P4I30_RS28440 (KAM622c_55690)	123218..123610	-	393	WP_047744999.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / qnrA1 / sul1 / dfrA19 / aph(3'')-Ib / aph(6)-Id	-	1..220409	220409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50155.10 Da
• Isoelectric Point: 6.1413

>T42476 WP_047744996.1 NZ_AP026394:c118874-117549 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKCHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAEHDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPPQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T42476 NZ_AP026394:c118874-117549 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGACGAACCCACAGATTTATATTCCGTGAAGTGTCACCCTCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAATGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGCATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCACTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGCGCAGAGC
ATGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCAATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACGGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGAGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCCGCAGCTTGCCGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10989.46 Da
• Isoelectric Point: 10.5348

>AT42476 WP_032636197.1 NZ_AP026394:c119172-118879 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTTSSERK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT42476 NZ_AP026394:c119172-118879 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA