Detailed information of TA system TA042475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 140986..141508
• Replicon: plasmid pKAM622_1
• Accession: NZ_AP026393
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: P4I30_RS27330
• Protein ID: WP_004181778.1
• Coordinates: 141224..141508 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: P4I30_RS27325
• Protein ID: WP_004181777.1
• Coordinates: 140986..141234 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I30_RS27300 (KAM622c_53170)	136534..136938	-	405	WP_276085848.1	hypothetical protein	-
P4I30_RS27305 (KAM622c_53180)	136983..137171	-	189	WP_276085849.1	hypothetical protein	-
P4I30_RS27310 (KAM622c_53190)	137720..137938	-	219	WP_276085850.1	hypothetical protein	-
P4I30_RS27315 (KAM622c_53200)	137935..138507	-	573	WP_276085851.1	hypothetical protein	-
P4I30_RS27320 (KAM622c_53220)	138908..140695	-	1788	WP_276085852.1	ATP-dependent helicase	-
P4I30_RS27325 (KAM622c_53230)	140986..141234	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
P4I30_RS27330 (KAM622c_53240)	141224..141508	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P4I30_RS27335	141525..141626	-	102	Protein_159	IS200/IS605 family transposase	-
P4I30_RS27340 (KAM622c_53250)	141662..142888	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
P4I30_RS27345 (KAM622c_53260)	143159..143383	-	225	Protein_161	transposase	-
P4I30_RS27350 (KAM622c_53270)	143462..143890	-	429	WP_077257669.1	IS200/IS605 family transposase	-
P4I30_RS27355 (KAM622c_53280)	143926..145113	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
P4I30_RS27360 (KAM622c_53290)	145158..145529	-	372	WP_040209644.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..222029	222029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T42475 WP_004181778.1 NZ_AP026393:141224-141508 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T42475 NZ_AP026393:141224-141508 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT42475 WP_004181777.1 NZ_AP026393:140986-141234 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT42475 NZ_AP026393:140986-141234 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8