Detailed information of TA system TA042473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4002651..4003269
• Replicon: chromosome
• Accession: NZ_AP026392
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: P4I30_RS20185
• Protein ID: WP_002892050.1
• Coordinates: 4003051..4003269 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: J2DPF6
• Locus tag: P4I30_RS20180
• Protein ID: WP_002892066.1
• Coordinates: 4002651..4003025 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I30_RS20165 (3997806)	3997806..3998999	+	1194	WP_023288645.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
P4I30_RS20170 (3999022)	3999022..4002168	+	3147	WP_065882810.1	multidrug efflux RND transporter permease subunit AcrB	-
P4I30_RS20175 (4002276)	4002276..4002572	+	297	WP_276085316.1	efflux RND transporter permease subunit	-
P4I30_RS20180 (4002651)	4002651..4003025	+	375	WP_002892066.1	Hha toxicity modulator TomB	Antitoxin
P4I30_RS20185 (4003051)	4003051..4003269	+	219	WP_002892050.1	HHA domain-containing protein	Toxin
P4I30_RS20190 (4003428)	4003428..4003994	+	567	WP_261901706.1	maltose O-acetyltransferase	-
P4I30_RS20195 (4003975)	4003975..4004097	-	123	WP_261901707.1	hypothetical protein	-
P4I30_RS20200 (4004131)	4004131..4004601	+	471	WP_023288642.1	YlaC family protein	-
P4I30_RS20205 (4004570)	4004570..4006027	-	1458	WP_261901708.1	PLP-dependent aminotransferase family protein	-
P4I30_RS20210 (4006128)	4006128..4006826	+	699	WP_261901709.1	GNAT family protein	-
P4I30_RS20215 (4006823)	4006823..4006963	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
P4I30_RS20220 (4006963)	4006963..4007226	-	264	WP_004204754.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T42473 WP_002892050.1 NZ_AP026392:4003051-4003269 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T42473 NZ_AP026392:4003051-4003269 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGCCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14368.06 Da
• Isoelectric Point: 4.8989

>AT42473 WP_002892066.1 NZ_AP026392:4002651-4003025 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT42473 NZ_AP026392:4002651-4003025 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACGTTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGATACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2

Antitoxin

• AlphaFold DB: A0A0H3GJ93