Detailed information of TA system TA042460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 290..837
• Replicon: plasmid pKAM471_3
• Accession: NZ_AP026373
• Organism: Aeromonas caviae strain KAM471

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: OCX46_RS22120
• Protein ID: WP_202212061.1
• Coordinates: 290..598 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: OCX46_RS22125
• Protein ID: WP_202212060.1
• Coordinates: 598..837 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX46_RS22115	1..5009	-	5009	WP_261921579.1	replication initiation protein	-
OCX46_RS22120 (KAM471c_42560)	290..598	-	309	WP_202212061.1	CcdB family protein	Toxin
OCX46_RS22125 (KAM471c_42570)	598..837	-	240	WP_202212060.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
OCX46_RS22130 (KAM471c_42580)	929..1411	-	483	WP_202212059.1	hypothetical protein	-
OCX46_RS22135 (KAM471c_42590)	1592..1900	-	309	WP_223918476.1	mobilization protein	-
OCX46_RS22140 (KAM471c_42600)	1901..2437	-	537	WP_223938704.1	DUF1640 domain-containing protein	-
OCX46_RS22145	2466..3092	-	627	Protein_6	relaxase/mobilization nuclease domain-containing protein	-
OCX46_RS22150 (KAM471c_42620)	3142..3552	-	411	WP_202212057.1	MobC family plasmid mobilization relaxosome protein	-
OCX46_RS22155 (KAM471c_42630)	3762..4052	+	291	WP_202212056.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5009	5009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11409.19 Da
• Isoelectric Point: 5.6642

>T42460 WP_202212061.1 NZ_AP026373:c598-290 [Aeromonas caviae]
MQFTIYRNKGNARDYPYLLNVQSDIIGELHTRMVIPLVSLADFPGRPAQRLNPVITVEGGKYLVLTHEMAAVRLAQLGEE
VTDVQESRQLIKGAIDFLLDGV

Nucleotide

• Length: 309 bp

>T42460 NZ_AP026373:c598-290 [Aeromonas caviae]
ATGCAGTTCACGATCTACCGCAACAAGGGCAATGCCCGCGACTACCCCTATTTGCTGAATGTGCAGAGCGACATCATCGG
CGAACTGCACACCCGGATGGTGATCCCCTTGGTCTCGCTGGCGGACTTCCCCGGACGGCCTGCACAGCGACTTAATCCGG
TGATCACCGTCGAGGGTGGCAAGTATCTGGTGTTAACCCACGAAATGGCGGCGGTGCGACTGGCGCAGCTGGGGGAGGAA
GTGACCGATGTTCAGGAGTCGCGTCAGCTCATCAAGGGGGCAATAGATTTCCTGCTGGATGGCGTGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8852.04 Da
• Isoelectric Point: 10.6724

>AT42460 WP_202212060.1 NZ_AP026373:c837-598 [Aeromonas caviae]
MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLATALQHEINGSEAERWKRENRAAIQTLNRMVEERGLFSDDHRTF

Nucleotide

• Length: 240 bp

>AT42460 NZ_AP026373:c837-598 [Aeromonas caviae]
ATGGTCGCAGCAGCAATGCGCAGTAAGAGAACGGTCAGCGTCACACTGGAGCCCAGCTTGCTGGAACGAGCCAGAGCGGC
GGGGATCAATCTGTCCGCCACTCTGGCTACCGCGCTACAGCATGAAATCAATGGCTCAGAGGCCGAGCGCTGGAAGCGTG
AGAACAGAGCCGCAATACAGACTCTTAACCGGATGGTCGAAGAACGCGGCCTGTTTTCTGACGATCACAGGACGTTTTGA