Detailed information of TA system TA042450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5228125..5228720
• Replicon: chromosome
• Accession: NZ_AP026118
• Organism: Escherichia coli strain CEC13004

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: OO879_RS26105
• Protein ID: WP_000239579.1
• Coordinates: 5228370..5228720 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: OO879_RS26100
• Protein ID: WP_001223210.1
• Coordinates: 5228125..5228376 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO879_RS26090 (5223790)	5223790..5227569	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
OO879_RS26095 (5227572)	5227572..5227913	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
OO879_RS26100 (5228125)	5228125..5228376	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
OO879_RS26105 (5228370)	5228370..5228720	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
OO879_RS26110 (5228800)	5228800..5229330	-	531	WP_000055075.1	inorganic diphosphatase	-
OO879_RS26115 (5229640)	5229640..5230596	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
OO879_RS26120 (5230736)	5230736..5232238	+	1503	Protein_5102	sugar ABC transporter ATP-binding protein	-
OO879_RS26125 (5232252)	5232252..5233274	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T42450 WP_000239579.1 NZ_AP026118:5228370-5228720 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T42450 NZ_AP026118:5228370-5228720 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT42450 WP_001223210.1 NZ_AP026118:5228125-5228376 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT42450 NZ_AP026118:5228125-5228376 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8