Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4295631..4296262 Replicon chromosome
Accession NZ_AP026118
Organism Escherichia coli strain CEC13004

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO879_RS21665 Protein ID WP_001259385.1
Coordinates 4295987..4296262 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO879_RS21660 Protein ID WP_000593555.1
Coordinates 4295631..4295990 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO879_RS21630 (4290743) 4290743..4291216 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO879_RS21635 (4291213) 4291213..4291494 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO879_RS21640 (4291571) 4291571..4292929 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO879_RS21645 (4292922) 4292922..4294430 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO879_RS21650 (4294420) 4294420..4294689 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO879_RS21655 (4294721) 4294721..4295581 + 861 WP_001131788.1 class II aldolase -
OO879_RS21660 (4295631) 4295631..4295990 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO879_RS21665 (4295987) 4295987..4296262 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO879_RS21670 (4296335) 4296335..4297459 - 1125 WP_001301659.1 ABC transporter permease -
OO879_RS21675 (4297459) 4297459..4300194 - 2736 WP_000149101.1 ribosome-associated ATPase/putative transporter RbbA -
OO879_RS21680 (4300191) 4300191..4301258 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42446 WP_001259385.1 NZ_AP026118:c4296262-4295987 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42446 NZ_AP026118:c4296262-4295987 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42446 WP_000593555.1 NZ_AP026118:c4295990-4295631 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42446 NZ_AP026118:c4295990-4295631 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References