Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3714747..3715401 Replicon chromosome
Accession NZ_AP026118
Organism Escherichia coli strain CEC13004

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag OO879_RS18680 Protein ID WP_000244781.1
Coordinates 3714747..3715154 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OO879_RS18685 Protein ID WP_000354046.1
Coordinates 3715135..3715401 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO879_RS18660 (3710704) 3710704..3712437 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
OO879_RS18665 (3712443) 3712443..3713153 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OO879_RS18670 (3713178) 3713178..3714074 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OO879_RS18675 (3714186) 3714186..3714707 + 522 WP_001055874.1 flavodoxin FldB -
OO879_RS18680 (3714747) 3714747..3715154 - 408 WP_000244781.1 protein YgfX Toxin
OO879_RS18685 (3715135) 3715135..3715401 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OO879_RS18690 (3715644) 3715644..3716624 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
OO879_RS18695 (3716701) 3716701..3717360 - 660 WP_000250274.1 hemolysin III family protein -
OO879_RS18700 (3717524) 3717524..3717835 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
OO879_RS18705 (3717880) 3717880..3719313 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T42441 WP_000244781.1 NZ_AP026118:c3715154-3714747 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T42441 NZ_AP026118:c3715154-3714747 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT42441 WP_000354046.1 NZ_AP026118:c3715401-3715135 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT42441 NZ_AP026118:c3715401-3715135 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References