Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2083655..2084126 Replicon chromosome
Accession NZ_AP026118
Organism Escherichia coli strain CEC13004

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag OO879_RS10305 Protein ID WP_001303511.1
Coordinates 2083848..2084126 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag OO879_RS10300 Protein ID WP_001302048.1
Coordinates 2083655..2083846 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO879_RS10265 (2079396) 2079396..2080136 - 741 WP_000788939.1 ATP-binding protein -
OO879_RS10270 (2080143) 2080143..2081105 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
OO879_RS10275 (2081128) 2081128..2081553 - 426 WP_000693943.1 toxin YdaT family protein -
OO879_RS10280 (2081550) 2081550..2081783 - 234 Protein_2016 transcriptional regulator -
OO879_RS10290 (2083094) 2083094..2083165 - 72 Protein_2018 hypothetical protein -
OO879_RS10295 (2083263) 2083263..2083634 + 372 WP_001169686.1 hypothetical protein -
OO879_RS10300 (2083655) 2083655..2083846 + 192 WP_001302048.1 hypothetical protein Antitoxin
OO879_RS10305 (2083848) 2083848..2084126 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO879_RS10310 (2084478) 2084478..2084777 + 300 WP_001240334.1 hypothetical protein -
OO879_RS10315 (2084849) 2084849..2085067 + 219 WP_001171930.1 protein YdfC -
OO879_RS10320 (2085636) 2085636..2085824 + 189 WP_000449192.1 cell division inhibition protein DicB -
OO879_RS10325 (2085821) 2085821..2086012 + 192 WP_001090200.1 DUF1482 family protein -
OO879_RS10330 (2086105) 2086105..2088576 + 2472 WP_000048458.1 exonuclease -
OO879_RS10335 (2088649) 2088649..2088900 + 252 WP_000005552.1 excisionase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 1992947..2099005 106058


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T42431 WP_001303511.1 NZ_AP026118:2083848-2084126 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T42431 NZ_AP026118:2083848-2084126 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT42431 WP_001302048.1 NZ_AP026118:2083655-2083846 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT42431 NZ_AP026118:2083655-2083846 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References