Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4274424..4275055 Replicon chromosome
Accession NZ_AP026116
Organism Escherichia coli strain CEC13002

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO850_RS21580 Protein ID WP_001259385.1
Coordinates 4274780..4275055 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO850_RS21575 Protein ID WP_000593555.1
Coordinates 4274424..4274783 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO850_RS21545 (4269536) 4269536..4270009 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO850_RS21550 (4270006) 4270006..4270287 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO850_RS21555 (4270364) 4270364..4271722 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO850_RS21560 (4271715) 4271715..4273223 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO850_RS21565 (4273213) 4273213..4273482 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO850_RS21570 (4273514) 4273514..4274374 + 861 WP_001131788.1 class II aldolase -
OO850_RS21575 (4274424) 4274424..4274783 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO850_RS21580 (4274780) 4274780..4275055 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO850_RS21585 (4275128) 4275128..4276252 - 1125 WP_001301659.1 ABC transporter permease -
OO850_RS21590 (4276252) 4276252..4278987 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO850_RS21595 (4278984) 4278984..4280051 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42407 WP_001259385.1 NZ_AP026116:c4275055-4274780 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42407 NZ_AP026116:c4275055-4274780 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42407 WP_000593555.1 NZ_AP026116:c4274783-4274424 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42407 NZ_AP026116:c4274783-4274424 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References