Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3692340..3692994 Replicon chromosome
Accession NZ_AP026116
Organism Escherichia coli strain CEC13002

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag OO850_RS18585 Protein ID WP_000244781.1
Coordinates 3692340..3692747 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OO850_RS18590 Protein ID WP_000354046.1
Coordinates 3692728..3692994 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO850_RS18565 (3688297) 3688297..3690030 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
OO850_RS18570 (3690036) 3690036..3690746 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OO850_RS18575 (3690771) 3690771..3691667 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OO850_RS18580 (3691779) 3691779..3692300 + 522 WP_001055874.1 flavodoxin FldB -
OO850_RS18585 (3692340) 3692340..3692747 - 408 WP_000244781.1 protein YgfX Toxin
OO850_RS18590 (3692728) 3692728..3692994 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OO850_RS18595 (3693237) 3693237..3694217 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
OO850_RS18600 (3694294) 3694294..3694953 - 660 WP_000250274.1 hemolysin III family protein -
OO850_RS18605 (3695117) 3695117..3695428 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
OO850_RS18610 (3695473) 3695473..3696906 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T42402 WP_000244781.1 NZ_AP026116:c3692747-3692340 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T42402 NZ_AP026116:c3692747-3692340 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT42402 WP_000354046.1 NZ_AP026116:c3692994-3692728 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT42402 NZ_AP026116:c3692994-3692728 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References