Detailed information of TA system TA042376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5136991..5137586
• Replicon: chromosome
• Accession: NZ_AP026114
• Organism: Escherichia coli strain CEC01302

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: OO829_RS25445
• Protein ID: WP_000239579.1
• Coordinates: 5137236..5137586 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: OO829_RS25440
• Protein ID: WP_001223210.1
• Coordinates: 5136991..5137242 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO829_RS25430 (5132656)	5132656..5136435	+	3780	WP_000060931.1	autotransporter assembly complex protein TamB	-
OO829_RS25435 (5136438)	5136438..5136779	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
OO829_RS25440 (5136991)	5136991..5137242	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
OO829_RS25445 (5137236)	5137236..5137586	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
OO829_RS25450 (5137666)	5137666..5138196	-	531	WP_000055075.1	inorganic diphosphatase	-
OO829_RS25455 (5138506)	5138506..5139462	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
OO829_RS25460 (5139602)	5139602..5141104	+	1503	Protein_4971	sugar ABC transporter ATP-binding protein	-
OO829_RS25465 (5141118)	5141118..5142140	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T42376 WP_000239579.1 NZ_AP026114:5137236-5137586 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T42376 NZ_AP026114:5137236-5137586 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT42376 WP_001223210.1 NZ_AP026114:5136991-5137242 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT42376 NZ_AP026114:5136991-5137242 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8