Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5040114..5040611 Replicon chromosome
Accession NZ_AP026114
Organism Escherichia coli strain CEC01302

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO829_RS24945 Protein ID WP_001173343.1
Coordinates 5040438..5040611 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO829_RS24940 Protein ID WP_000398619.1
Coordinates 5040114..5040410 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO829_RS24920 (5036845) 5036845..5037564 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO829_RS24925 (5037561) 5037561..5039192 - 1632 WP_001216477.1 sensor histidine kinase -
OO829_RS24930 (5039373) 5039373..5039603 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO829_RS24935 (5039615) 5039615..5039887 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO829_RS24940 (5040114) 5040114..5040410 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO829_RS24945 (5040438) 5040438..5040611 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO829_RS24950 (5040730) 5040730..5042247 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO829_RS24955 (5042484) 5042484..5043941 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42375 WP_001173343.1 NZ_AP026114:5040438-5040611 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42375 NZ_AP026114:5040438-5040611 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42375 WP_000398619.1 NZ_AP026114:5040114-5040410 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42375 NZ_AP026114:5040114-5040410 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References