Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4220458..4221089 Replicon chromosome
Accession NZ_AP026114
Organism Escherichia coli strain CEC01302

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO829_RS21115 Protein ID WP_001259385.1
Coordinates 4220814..4221089 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO829_RS21110 Protein ID WP_000593555.1
Coordinates 4220458..4220817 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO829_RS21080 (4215570) 4215570..4216043 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO829_RS21085 (4216040) 4216040..4216321 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO829_RS21090 (4216398) 4216398..4217756 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO829_RS21095 (4217749) 4217749..4219257 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO829_RS21100 (4219247) 4219247..4219516 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO829_RS21105 (4219548) 4219548..4220408 + 861 WP_001131788.1 class II aldolase -
OO829_RS21110 (4220458) 4220458..4220817 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO829_RS21115 (4220814) 4220814..4221089 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO829_RS21120 (4221162) 4221162..4222286 - 1125 WP_001301659.1 ABC transporter permease -
OO829_RS21125 (4222286) 4222286..4225021 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO829_RS21130 (4225018) 4225018..4226085 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42372 WP_001259385.1 NZ_AP026114:c4221089-4220814 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42372 NZ_AP026114:c4221089-4220814 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42372 WP_000593555.1 NZ_AP026114:c4220817-4220458 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42372 NZ_AP026114:c4220817-4220458 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References