Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 549992..550610 Replicon chromosome
Accession NZ_AP026114
Organism Escherichia coli strain CEC01302

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OO829_RS02570 Protein ID WP_001291435.1
Coordinates 549992..550210 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OO829_RS02575 Protein ID WP_000344800.1
Coordinates 550236..550610 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO829_RS02535 (545279) 545279..545851 + 573 WP_000779831.1 YbaY family lipoprotein -
OO829_RS02540 (545884) 545884..546195 - 312 WP_000409911.1 MGMT family protein -
OO829_RS02550 (546574) 546574..546927 + 354 WP_000878140.1 DUF1428 domain-containing protein -
OO829_RS02555 (546969) 546969..548519 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OO829_RS02560 (548683) 548683..549153 - 471 WP_000136192.1 YlaC family protein -
OO829_RS02565 (549269) 549269..549820 - 552 WP_000102553.1 maltose O-acetyltransferase -
OO829_RS02570 (549992) 549992..550210 - 219 WP_001291435.1 HHA domain-containing protein Toxin
OO829_RS02575 (550236) 550236..550610 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OO829_RS02580 (551156) 551156..554305 - 3150 WP_264831084.1 efflux RND transporter permease AcrB -
OO829_RS02585 (554328) 554328..555521 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42352 WP_001291435.1 NZ_AP026114:c550210-549992 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42352 NZ_AP026114:c550210-549992 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42352 WP_000344800.1 NZ_AP026114:c550610-550236 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42352 NZ_AP026114:c550610-550236 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References