Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 2326..2885 Replicon plasmid pCEC03102_1
Accession NZ_AP026113
Organism Escherichia coli strain CEC03102

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag OO877_RS27310 Protein ID WP_000421248.1
Coordinates 2610..2885 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag OO877_RS27305 Protein ID WP_001178089.1
Coordinates 2326..2610 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO877_RS27290 (1) 1..255 + 255 WP_000083831.1 replication regulatory protein RepA -
OO877_RS27295 (491) 491..565 + 75 WP_001370046.1 RepA leader peptide Tap -
OO877_RS27300 (558) 558..1415 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
OO877_RS27305 (2326) 2326..2610 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
OO877_RS27310 (2610) 2610..2885 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO877_RS27315 (2980) 2980..3186 + 207 WP_001105064.1 hypothetical protein -
OO877_RS27320 (3286) 3286..3501 - 216 Protein_6 transposase -
OO877_RS27325 (3545) 3545..4111 - 567 Protein_7 IS3-like element IS600 family transposase -
OO877_RS27330 (4111) 4111..4527 - 417 Protein_8 IS91 family transposase -
OO877_RS27335 (4527) 4527..4712 - 186 WP_001302179.1 hypothetical protein -
OO877_RS27340 (4889) 4889..5524 + 636 Protein_10 catalase/peroxidase KatP -
OO877_RS27345 (5580) 5580..6793 + 1214 WP_162829202.1 IS3-like element IS1203 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / hlyA / hlyB / hlyD / toxB 1..72140 72140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T42348 WP_000421248.1 NZ_AP026113:2610-2885 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T42348 NZ_AP026113:2610-2885 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT42348 WP_001178089.1 NZ_AP026113:2326-2610 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT42348 NZ_AP026113:2326-2610 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References