Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4370552..4371183 Replicon chromosome
Accession NZ_AP026112
Organism Escherichia coli strain CEC03102

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO877_RS22150 Protein ID WP_001259385.1
Coordinates 4370908..4371183 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO877_RS22145 Protein ID WP_000593555.1
Coordinates 4370552..4370911 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO877_RS22115 (4365664) 4365664..4366137 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO877_RS22120 (4366134) 4366134..4366415 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO877_RS22125 (4366492) 4366492..4367850 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO877_RS22130 (4367843) 4367843..4369351 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO877_RS22135 (4369341) 4369341..4369610 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO877_RS22140 (4369642) 4369642..4370502 + 861 WP_001131788.1 class II aldolase -
OO877_RS22145 (4370552) 4370552..4370911 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO877_RS22150 (4370908) 4370908..4371183 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO877_RS22155 (4371256) 4371256..4372380 - 1125 WP_001301659.1 ABC transporter permease -
OO877_RS22160 (4372380) 4372380..4375115 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO877_RS22165 (4375112) 4375112..4376179 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42339 WP_001259385.1 NZ_AP026112:c4371183-4370908 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42339 NZ_AP026112:c4371183-4370908 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42339 WP_000593555.1 NZ_AP026112:c4370911-4370552 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42339 NZ_AP026112:c4370911-4370552 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References