Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4269052..4269683 Replicon chromosome
Accession NZ_AP026110
Organism Escherichia coli strain CEC08123

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO855_RS21470 Protein ID WP_001259385.1
Coordinates 4269408..4269683 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO855_RS21465 Protein ID WP_000593555.1
Coordinates 4269052..4269411 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO855_RS21435 (4264165) 4264165..4264638 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO855_RS21440 (4264635) 4264635..4264916 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO855_RS21445 (4264993) 4264993..4266350 + 1358 Protein_4202 PTS galactitol transporter subunit IIC -
OO855_RS21450 (4266343) 4266343..4267851 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO855_RS21455 (4267841) 4267841..4268110 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO855_RS21460 (4268142) 4268142..4269002 + 861 WP_001131788.1 class II aldolase -
OO855_RS21465 (4269052) 4269052..4269411 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO855_RS21470 (4269408) 4269408..4269683 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO855_RS21475 (4269756) 4269756..4270880 - 1125 WP_001301659.1 ABC transporter permease -
OO855_RS21480 (4270880) 4270880..4273615 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO855_RS21485 (4273612) 4273612..4274679 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42300 WP_001259385.1 NZ_AP026110:c4269683-4269408 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42300 NZ_AP026110:c4269683-4269408 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42300 WP_000593555.1 NZ_AP026110:c4269411-4269052 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42300 NZ_AP026110:c4269411-4269052 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References