Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4420886..4421517 Replicon chromosome
Accession NZ_AP026108
Organism Escherichia coli strain CEC13091

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO842_RS22380 Protein ID WP_001259385.1
Coordinates 4421242..4421517 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO842_RS22375 Protein ID WP_000593555.1
Coordinates 4420886..4421245 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO842_RS22345 (4415998) 4415998..4416471 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO842_RS22350 (4416468) 4416468..4416749 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO842_RS22355 (4416826) 4416826..4418184 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO842_RS22360 (4418177) 4418177..4419685 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO842_RS22365 (4419675) 4419675..4419944 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO842_RS22370 (4419976) 4419976..4420836 + 861 WP_001131788.1 class II aldolase -
OO842_RS22375 (4420886) 4420886..4421245 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO842_RS22380 (4421242) 4421242..4421517 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO842_RS22385 (4421590) 4421590..4422714 - 1125 WP_001301659.1 ABC transporter permease -
OO842_RS22390 (4422714) 4422714..4425449 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO842_RS22395 (4425446) 4425446..4426513 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42263 WP_001259385.1 NZ_AP026108:c4421517-4421242 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42263 NZ_AP026108:c4421517-4421242 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42263 WP_000593555.1 NZ_AP026108:c4421245-4420886 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42263 NZ_AP026108:c4421245-4420886 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References