Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 548721..549339 Replicon chromosome
Accession NZ_AP026108
Organism Escherichia coli strain CEC13091

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OO842_RS02560 Protein ID WP_001291435.1
Coordinates 548721..548939 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OO842_RS02565 Protein ID WP_000344800.1
Coordinates 548965..549339 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO842_RS02525 (544008) 544008..544580 + 573 WP_000779831.1 YbaY family lipoprotein -
OO842_RS02530 (544613) 544613..544924 - 312 WP_000409911.1 MGMT family protein -
OO842_RS02540 (545303) 545303..545656 + 354 WP_000878140.1 DUF1428 domain-containing protein -
OO842_RS02545 (545698) 545698..547248 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OO842_RS02550 (547412) 547412..547882 - 471 WP_000136192.1 YlaC family protein -
OO842_RS02555 (547998) 547998..548549 - 552 WP_000102553.1 maltose O-acetyltransferase -
OO842_RS02560 (548721) 548721..548939 - 219 WP_001291435.1 HHA domain-containing protein Toxin
OO842_RS02565 (548965) 548965..549339 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OO842_RS02570 (549885) 549885..553034 - 3150 WP_001132452.1 efflux RND transporter permease AcrB -
OO842_RS02575 (553057) 553057..554250 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42236 WP_001291435.1 NZ_AP026108:c548939-548721 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42236 NZ_AP026108:c548939-548721 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42236 WP_000344800.1 NZ_AP026108:c549339-548965 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42236 NZ_AP026108:c549339-548965 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References