Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5032853..5033350 Replicon chromosome
Accession NZ_AP026106
Organism Escherichia coli strain CEC01311

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO831_RS24815 Protein ID WP_001173343.1
Coordinates 5033177..5033350 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO831_RS24810 Protein ID WP_000398619.1
Coordinates 5032853..5033149 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO831_RS24790 (5029584) 5029584..5030303 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO831_RS24795 (5030300) 5030300..5031931 - 1632 WP_001216477.1 sensor histidine kinase -
OO831_RS24800 (5032112) 5032112..5032342 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO831_RS24805 (5032354) 5032354..5032626 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO831_RS24810 (5032853) 5032853..5033149 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO831_RS24815 (5033177) 5033177..5033350 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO831_RS24820 (5033469) 5033469..5034986 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO831_RS24825 (5035223) 5035223..5036680 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42226 WP_001173343.1 NZ_AP026106:5033177-5033350 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42226 NZ_AP026106:5033177-5033350 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42226 WP_000398619.1 NZ_AP026106:5032853-5033149 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42226 NZ_AP026106:5032853-5033149 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References