Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4224051..4224682 Replicon chromosome
Accession NZ_AP026106
Organism Escherichia coli strain CEC01311

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO831_RS21085 Protein ID WP_001259385.1
Coordinates 4224407..4224682 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO831_RS21080 Protein ID WP_000593555.1
Coordinates 4224051..4224410 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO831_RS21050 (4219163) 4219163..4219636 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO831_RS21055 (4219633) 4219633..4219914 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO831_RS21060 (4219991) 4219991..4221349 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO831_RS21065 (4221342) 4221342..4222850 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO831_RS21070 (4222840) 4222840..4223109 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO831_RS21075 (4223141) 4223141..4224001 + 861 WP_001131788.1 class II aldolase -
OO831_RS21080 (4224051) 4224051..4224410 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO831_RS21085 (4224407) 4224407..4224682 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO831_RS21090 (4224755) 4224755..4225879 - 1125 WP_001301659.1 ABC transporter permease -
OO831_RS21095 (4225879) 4225879..4228614 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO831_RS21100 (4228611) 4228611..4229678 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42223 WP_001259385.1 NZ_AP026106:c4224682-4224407 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42223 NZ_AP026106:c4224682-4224407 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42223 WP_000593555.1 NZ_AP026106:c4224410-4224051 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42223 NZ_AP026106:c4224410-4224051 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References