Detailed information of TA system TA042162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 27506..28150
• Replicon: plasmid pNIID93_2
• Accession: NZ_AP026103
• Organism: Escherichia coli strain 93_161312

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7L6LII1
• Locus tag: OO868_RS28205
• Protein ID: WP_000833471.1
• Coordinates: 27968..28150 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7L6LHN8
• Locus tag: OO868_RS28200
• Protein ID: WP_000466317.1
• Coordinates: 27506..27943 (-)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO868_RS28180 (CL8NIID93_54160)	24917..25252	-	336	WP_000963830.1	DUF6290 family protein	-
OO868_RS28185 (CL8NIID93_54170)	25598..26005	+	408	WP_001190619.1	hypothetical protein	-
OO868_RS28190 (CL8NIID93_54180)	26015..26776	+	762	WP_001097937.1	conjugal transfer protein TraL	-
OO868_RS28195 (CL8NIID93_54190)	26773..27477	+	705	WP_001279868.1	hypothetical protein	-
OO868_RS28200 (CL8NIID93_54200)	27506..27943	-	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
OO868_RS28205	27968..28150	-	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	Toxin
OO868_RS28210 (CL8NIID93_54220)	28580..28858	-	279	WP_000545934.1	hypothetical protein	-
OO868_RS28215 (CL8NIID93_54230)	28916..29245	-	330	WP_000449469.1	hypothetical protein	-
OO868_RS28220 (CL8NIID93_54240)	29685..29927	-	243	WP_001450803.1	hypothetical protein	-
OO868_RS28225 (CL8NIID93_54250)	29917..30183	-	267	WP_000868275.1	hypothetical protein	-
OO868_RS28230 (CL8NIID93_54260)	30234..30458	-	225	WP_000749827.1	hypothetical protein	-
OO868_RS28235 (CL8NIID93_54270)	30554..30778	-	225	WP_000866040.1	hypothetical protein	-
OO868_RS28240 (CL8NIID93_54280)	30819..31142	-	324	WP_000432877.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32822	32822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-138)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6675.97 Da
• Isoelectric Point: 11.0013

>T42162 WP_000833471.1 NZ_AP026103:c28150-27968 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Nucleotide

• Length: 183 bp

>T42162 NZ_AP026103:c28150-27968 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16227.10 Da
• Isoelectric Point: 4.3135

>AT42162 WP_000466317.1 NZ_AP026103:c27943-27506 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Nucleotide

• Length: 438 bp

>AT42162 NZ_AP026103:c27943-27506 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Structures

Toxin

• AlphaFold DB: A0A7L6LII1

Antitoxin

• AlphaFold DB: A0A7L6LHN8