Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 27506..28150 Replicon plasmid pNIID93_2
Accession NZ_AP026103
Organism Escherichia coli strain 93_161312

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag OO868_RS28205 Protein ID WP_000833471.1
Coordinates 27968..28150 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag OO868_RS28200 Protein ID WP_000466317.1
Coordinates 27506..27943 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO868_RS28180 (CL8NIID93_54160) 24917..25252 - 336 WP_000963830.1 DUF6290 family protein -
OO868_RS28185 (CL8NIID93_54170) 25598..26005 + 408 WP_001190619.1 hypothetical protein -
OO868_RS28190 (CL8NIID93_54180) 26015..26776 + 762 WP_001097937.1 conjugal transfer protein TraL -
OO868_RS28195 (CL8NIID93_54190) 26773..27477 + 705 WP_001279868.1 hypothetical protein -
OO868_RS28200 (CL8NIID93_54200) 27506..27943 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO868_RS28205 27968..28150 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
OO868_RS28210 (CL8NIID93_54220) 28580..28858 - 279 WP_000545934.1 hypothetical protein -
OO868_RS28215 (CL8NIID93_54230) 28916..29245 - 330 WP_000449469.1 hypothetical protein -
OO868_RS28220 (CL8NIID93_54240) 29685..29927 - 243 WP_001450803.1 hypothetical protein -
OO868_RS28225 (CL8NIID93_54250) 29917..30183 - 267 WP_000868275.1 hypothetical protein -
OO868_RS28230 (CL8NIID93_54260) 30234..30458 - 225 WP_000749827.1 hypothetical protein -
OO868_RS28235 (CL8NIID93_54270) 30554..30778 - 225 WP_000866040.1 hypothetical protein -
OO868_RS28240 (CL8NIID93_54280) 30819..31142 - 324 WP_000432877.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32822 32822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T42162 WP_000833471.1 NZ_AP026103:c28150-27968 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T42162 NZ_AP026103:c28150-27968 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT42162 WP_000466317.1 NZ_AP026103:c27943-27506 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT42162 NZ_AP026103:c27943-27506 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References