Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5222812..5223309 Replicon chromosome
Accession NZ_AP026101
Organism Escherichia coli strain 93_161312

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO868_RS26255 Protein ID WP_001173343.1
Coordinates 5223136..5223309 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO868_RS26250 Protein ID WP_000398619.1
Coordinates 5222812..5223108 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO868_RS26230 (5219543) 5219543..5220262 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO868_RS26235 (5220259) 5220259..5221890 - 1632 WP_001216477.1 sensor histidine kinase -
OO868_RS26240 (5222071) 5222071..5222301 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO868_RS26245 (5222313) 5222313..5222585 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO868_RS26250 (5222812) 5222812..5223108 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO868_RS26255 (5223136) 5223136..5223309 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO868_RS26260 (5223428) 5223428..5224945 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO868_RS26265 (5225182) 5225182..5226639 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42154 WP_001173343.1 NZ_AP026101:5223136-5223309 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42154 NZ_AP026101:5223136-5223309 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42154 WP_000398619.1 NZ_AP026101:5222812-5223108 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42154 NZ_AP026101:5222812-5223108 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References