Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4403094..4403725 Replicon chromosome
Accession NZ_AP026101
Organism Escherichia coli strain 93_161312

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO868_RS22425 Protein ID WP_001259385.1
Coordinates 4403450..4403725 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO868_RS22420 Protein ID WP_000593555.1
Coordinates 4403094..4403453 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO868_RS22390 (4398206) 4398206..4398679 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO868_RS22395 (4398676) 4398676..4398957 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO868_RS22400 (4399034) 4399034..4400392 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO868_RS22405 (4400385) 4400385..4401893 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO868_RS22410 (4401883) 4401883..4402152 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO868_RS22415 (4402184) 4402184..4403044 + 861 WP_001131788.1 class II aldolase -
OO868_RS22420 (4403094) 4403094..4403453 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO868_RS22425 (4403450) 4403450..4403725 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO868_RS22430 (4403798) 4403798..4404922 - 1125 WP_001301659.1 ABC transporter permease -
OO868_RS22435 (4404922) 4404922..4407657 - 2736 WP_000149101.1 ribosome-associated ATPase/putative transporter RbbA -
OO868_RS22440 (4407654) 4407654..4408721 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42151 WP_001259385.1 NZ_AP026101:c4403725-4403450 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42151 NZ_AP026101:c4403725-4403450 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42151 WP_000593555.1 NZ_AP026101:c4403453-4403094 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42151 NZ_AP026101:c4403453-4403094 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References