Detailed information of TA system TA042149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4311610..4312364
• Replicon: chromosome
• Accession: NZ_AP026101
• Organism: Escherichia coli strain 93_161312

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: OO868_RS21965
• Protein ID: WP_001301452.1
• Coordinates: 4311879..4312364 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: OO868_RS21960
• Protein ID: WP_000801912.1
• Coordinates: 4311610..4311888 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO868_RS21955 (4308838)	4308838..4311543	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
OO868_RS21960 (4311610)	4311610..4311888	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
OO868_RS21965 (4311879)	4311879..4312364	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
OO868_RS21970 (4312414)	4312414..4313442	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
OO868_RS21975 (4313446)	4313446..4314672	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
OO868_RS21980 (4314860)	4314860..4316458	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
OO868_RS21985 (4316440)	4316440..4317198	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T42149 WP_001301452.1 NZ_AP026101:4311879-4312364 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T42149 NZ_AP026101:4311879-4312364 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT42149 WP_000801912.1 NZ_AP026101:4311610-4311888 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT42149 NZ_AP026101:4311610-4311888 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9