Detailed information of TA system TA042143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1873292..1873930
• Replicon: chromosome
• Accession: NZ_AP026101
• Organism: Escherichia coli strain 93_161312

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: OO868_RS09200
• Protein ID: WP_000813794.1
• Coordinates: 1873754..1873930 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: OO868_RS09195
• Protein ID: WP_001270286.1
• Coordinates: 1873292..1873708 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO868_RS09175 (1868444)	1868444..1869385	-	942	WP_001251315.1	ABC transporter permease	-
OO868_RS09180 (1869386)	1869386..1870399	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
OO868_RS09185 (1870417)	1870417..1871562	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
OO868_RS09190 (1871807)	1871807..1873213	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
OO868_RS09195 (1873292)	1873292..1873708	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
OO868_RS09200 (1873754)	1873754..1873930	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
OO868_RS09205 (1874152)	1874152..1874382	+	231	WP_000494244.1	YncJ family protein	-
OO868_RS09210 (1874474)	1874474..1876435	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
OO868_RS09215 (1876508)	1876508..1877044	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
OO868_RS09220 (1877136)	1877136..1878309	+	1174	Protein_1807	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T42143 WP_000813794.1 NZ_AP026101:c1873930-1873754 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T42143 NZ_AP026101:c1873930-1873754 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT42143 WP_001270286.1 NZ_AP026101:c1873708-1873292 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT42143 NZ_AP026101:c1873708-1873292 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB