Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1649569..1650040 Replicon chromosome
Accession NZ_AP026101
Organism Escherichia coli strain 93_161312

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag OO868_RS07985 Protein ID WP_001303511.1
Coordinates 1649569..1649847 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag OO868_RS07990 Protein ID WP_001302048.1
Coordinates 1649849..1650040 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO868_RS07960 (1646505) 1646505..1647590 - 1086 Protein_1561 exonuclease -
OO868_RS07965 (1647683) 1647683..1647874 - 192 WP_001090200.1 DUF1482 family protein -
OO868_RS07970 (1647871) 1647871..1648059 - 189 WP_000449192.1 cell division inhibition protein DicB -
OO868_RS07975 (1648628) 1648628..1648846 - 219 WP_001171930.1 protein YdfC -
OO868_RS07980 (1648918) 1648918..1649217 - 300 WP_001240334.1 hypothetical protein -
OO868_RS07985 (1649569) 1649569..1649847 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO868_RS07990 (1649849) 1649849..1650040 - 192 WP_001302048.1 hypothetical protein Antitoxin
OO868_RS07995 (1650061) 1650061..1650432 - 372 WP_001169686.1 hypothetical protein -
OO868_RS08000 (1650530) 1650530..1650601 + 72 Protein_1569 hypothetical protein -
OO868_RS08005 (1650654) 1650654..1651867 + 1214 WP_162829202.1 IS3-like element IS1203 family transposase -
OO868_RS08010 (1651912) 1651912..1652145 + 234 Protein_1571 transcriptional regulator -
OO868_RS08015 (1652142) 1652142..1652567 + 426 WP_000693943.1 toxin YdaT family protein -
OO868_RS08020 (1652590) 1652590..1653552 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
OO868_RS08025 (1653559) 1653559..1654299 + 741 WP_000788939.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 1641096..1740758 99662
- inside IScluster/Tn - - 1645241..1651867 6626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T42140 WP_001303511.1 NZ_AP026101:c1649847-1649569 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T42140 NZ_AP026101:c1649847-1649569 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT42140 WP_001302048.1 NZ_AP026101:c1650040-1649849 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT42140 NZ_AP026101:c1650040-1649849 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References