Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5348165..5348662 Replicon chromosome
Accession NZ_AP026099
Organism Escherichia coli strain 61_150228

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO862_RS27115 Protein ID WP_001173343.1
Coordinates 5348489..5348662 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO862_RS27110 Protein ID WP_000398619.1
Coordinates 5348165..5348461 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO862_RS27090 (5344896) 5344896..5345615 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO862_RS27095 (5345612) 5345612..5347243 - 1632 WP_264831421.1 sensor histidine kinase -
OO862_RS27100 (5347424) 5347424..5347654 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO862_RS27105 (5347666) 5347666..5347938 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO862_RS27110 (5348165) 5348165..5348461 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO862_RS27115 (5348489) 5348489..5348662 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO862_RS27120 (5348781) 5348781..5350298 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO862_RS27125 (5350535) 5350535..5351992 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42121 WP_001173343.1 NZ_AP026099:5348489-5348662 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42121 NZ_AP026099:5348489-5348662 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42121 WP_000398619.1 NZ_AP026099:5348165-5348461 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42121 NZ_AP026099:5348165-5348461 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References