Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 2327..2886 Replicon plasmid pNIID57_1
Accession NZ_AP026098
Organism Escherichia coli strain 57_142493

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag OO832_RS27430 Protein ID WP_000421248.1
Coordinates 2611..2886 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag OO832_RS27425 Protein ID WP_001178089.1
Coordinates 2327..2611 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO832_RS27410 (1) 1..255 + 255 WP_000083831.1 replication regulatory protein RepA -
OO832_RS27415 (491) 491..565 + 75 WP_001370046.1 RepA leader peptide Tap -
OO832_RS27420 (558) 558..1415 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
OO832_RS27425 (2327) 2327..2611 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
OO832_RS27430 (2611) 2611..2886 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO832_RS27435 (2981) 2981..3187 + 207 WP_001105064.1 hypothetical protein -
OO832_RS27440 (3287) 3287..3502 - 216 Protein_6 transposase -
OO832_RS27445 (3546) 3546..4112 - 567 Protein_7 IS3-like element IS600 family transposase -
OO832_RS27450 (4112) 4112..4528 - 417 Protein_8 IS91 family transposase -
OO832_RS27455 (4528) 4528..4713 - 186 WP_001302179.1 hypothetical protein -
OO832_RS27460 (4890) 4890..7100 + 2211 WP_000592771.1 catalase/peroxidase KatP -
OO832_RS27465 (7144) 7144..7533 + 390 WP_001172748.1 cytochrome b562 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / toxB 1..57471 57471
- inside IScluster/Tn - espP 3546..19373 15827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T42094 WP_000421248.1 NZ_AP026098:2611-2886 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T42094 NZ_AP026098:2611-2886 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT42094 WP_001178089.1 NZ_AP026098:2327-2611 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT42094 NZ_AP026098:2327-2611 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References