Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4359330..4359961 Replicon chromosome
Accession NZ_AP026097
Organism Escherichia coli strain 57_142493

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO832_RS22140 Protein ID WP_001259385.1
Coordinates 4359686..4359961 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO832_RS22135 Protein ID WP_000593555.1
Coordinates 4359330..4359689 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO832_RS22105 (4354442) 4354442..4354915 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO832_RS22110 (4354912) 4354912..4355193 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO832_RS22115 (4355270) 4355270..4356628 + 1359 Protein_4331 PTS galactitol transporter subunit IIC -
OO832_RS22120 (4356621) 4356621..4358129 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO832_RS22125 (4358119) 4358119..4358388 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO832_RS22130 (4358420) 4358420..4359280 + 861 WP_001131788.1 class II aldolase -
OO832_RS22135 (4359330) 4359330..4359689 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO832_RS22140 (4359686) 4359686..4359961 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO832_RS22145 (4360034) 4360034..4361158 - 1125 WP_001301659.1 ABC transporter permease -
OO832_RS22150 (4361158) 4361158..4363893 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO832_RS22155 (4363890) 4363890..4364957 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T42085 WP_001259385.1 NZ_AP026097:c4359961-4359686 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T42085 NZ_AP026097:c4359961-4359686 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42085 WP_000593555.1 NZ_AP026097:c4359689-4359330 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42085 NZ_AP026097:c4359689-4359330 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References