Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 549973..550591 Replicon chromosome
Accession NZ_AP026097
Organism Escherichia coli strain 57_142493

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OO832_RS02565 Protein ID WP_001291435.1
Coordinates 549973..550191 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OO832_RS02570 Protein ID WP_000344800.1
Coordinates 550217..550591 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO832_RS02530 (545260) 545260..545832 + 573 WP_000779831.1 YbaY family lipoprotein -
OO832_RS02535 (545865) 545865..546176 - 312 WP_000409911.1 MGMT family protein -
OO832_RS02545 (546555) 546555..546908 + 354 WP_000878140.1 DUF1428 domain-containing protein -
OO832_RS02550 (546950) 546950..548500 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OO832_RS02555 (548664) 548664..549134 - 471 WP_000136192.1 YlaC family protein -
OO832_RS02560 (549250) 549250..549801 - 552 WP_000102553.1 maltose O-acetyltransferase -
OO832_RS02565 (549973) 549973..550191 - 219 WP_001291435.1 HHA domain-containing protein Toxin
OO832_RS02570 (550217) 550217..550591 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OO832_RS02575 (551137) 551137..554286 - 3150 WP_001132452.1 efflux RND transporter permease AcrB -
OO832_RS02580 (554309) 554309..555502 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42063 WP_001291435.1 NZ_AP026097:c550191-549973 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42063 NZ_AP026097:c550191-549973 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42063 WP_000344800.1 NZ_AP026097:c550591-550217 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42063 NZ_AP026097:c550591-550217 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References