Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2743082..2743720 Replicon chromosome
Accession NZ_AP026095
Organism Escherichia coli strain 53_142304

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag OO816_RS13840 Protein ID WP_000813794.1
Coordinates 2743544..2743720 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag OO816_RS13835 Protein ID WP_001270286.1
Coordinates 2743082..2743498 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO816_RS13815 (2738234) 2738234..2739175 - 942 WP_001251315.1 ABC transporter permease -
OO816_RS13820 (2739176) 2739176..2740189 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
OO816_RS13825 (2740207) 2740207..2741352 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
OO816_RS13830 (2741597) 2741597..2743003 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
OO816_RS13835 (2743082) 2743082..2743498 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
OO816_RS13840 (2743544) 2743544..2743720 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
OO816_RS13845 (2743942) 2743942..2744172 + 231 WP_000494244.1 YncJ family protein -
OO816_RS13850 (2744264) 2744264..2746225 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
OO816_RS13855 (2746298) 2746298..2746834 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
OO816_RS13860 (2746926) 2746926..2748087 + 1162 Protein_2715 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T42044 WP_000813794.1 NZ_AP026095:c2743720-2743544 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T42044 NZ_AP026095:c2743720-2743544 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT42044 WP_001270286.1 NZ_AP026095:c2743498-2743082 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT42044 NZ_AP026095:c2743498-2743082 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References