Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 924933..925587 Replicon chromosome
Accession NZ_AP026095
Organism Escherichia coli strain 53_142304

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag OO816_RS04435 Protein ID WP_000244781.1
Coordinates 925180..925587 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OO816_RS04430 Protein ID WP_000354046.1
Coordinates 924933..925199 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO816_RS04410 (921021) 921021..922454 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
OO816_RS04415 (922499) 922499..922810 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
OO816_RS04420 (922974) 922974..923633 + 660 WP_000250274.1 hemolysin III family protein -
OO816_RS04425 (923710) 923710..924690 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
OO816_RS04430 (924933) 924933..925199 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OO816_RS04435 (925180) 925180..925587 + 408 WP_000244781.1 protein YgfX Toxin
OO816_RS04440 (925627) 925627..926148 - 522 WP_001055874.1 flavodoxin FldB -
OO816_RS04445 (926260) 926260..927156 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OO816_RS04450 (927181) 927181..927891 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OO816_RS04455 (927897) 927897..929630 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T42033 WP_000244781.1 NZ_AP026095:925180-925587 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T42033 NZ_AP026095:925180-925587 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT42033 WP_000354046.1 NZ_AP026095:924933-925199 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT42033 NZ_AP026095:924933-925199 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References