Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 551253..551871 Replicon chromosome
Accession NZ_AP026095
Organism Escherichia coli strain 53_142304

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OO816_RS02570 Protein ID WP_001291435.1
Coordinates 551253..551471 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OO816_RS02575 Protein ID WP_000344800.1
Coordinates 551497..551871 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO816_RS02535 (546540) 546540..547112 + 573 WP_000779831.1 YbaY family lipoprotein -
OO816_RS02540 (547145) 547145..547456 - 312 WP_000409911.1 MGMT family protein -
OO816_RS02550 (547835) 547835..548188 + 354 WP_000878140.1 DUF1428 domain-containing protein -
OO816_RS02555 (548230) 548230..549780 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OO816_RS02560 (549944) 549944..550414 - 471 WP_000136192.1 YlaC family protein -
OO816_RS02565 (550530) 550530..551081 - 552 WP_000102553.1 maltose O-acetyltransferase -
OO816_RS02570 (551253) 551253..551471 - 219 WP_001291435.1 HHA domain-containing protein Toxin
OO816_RS02575 (551497) 551497..551871 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OO816_RS02580 (552417) 552417..555566 - 3150 WP_264831396.1 efflux RND transporter permease AcrB -
OO816_RS02585 (555578) 555578..556735 - 1158 WP_000160336.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42030 WP_001291435.1 NZ_AP026095:c551471-551253 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42030 NZ_AP026095:c551471-551253 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42030 WP_000344800.1 NZ_AP026095:c551871-551497 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42030 NZ_AP026095:c551871-551497 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References